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 Application Notes
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Removal of Endotoxin, RNase, DNase and Bacteria using the PURELAB Option-Q fitted with a Biofilter
 20 May 2008: The Biofilter, when fitted to the Option-Q, is very effective for producing water which is free from biologically active impurities. This makes it suitable for use with applications which require endotoxin free ultra-pure water, bacteria free water, and nuclease free ultra-pure water. This technology note explains several experimental challenges to determine endotoxin, DNase and RNase, TOC and resistivity, and bacteria levels in ultra-pure water from the Option-Q.
ELGA LabWater
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Endotoxin-Free Water for Tissue Culture
 05 Feb 2008: Water is a ubiquitous solvent used throughout cell isolation and culture. Therefore, water contamination may affect experimental outcomes. The use of ultrapure water is advisable for culturing of cells sensitive to environmental contaminants, such as endotoxins and bacteria. This application note presents cardiomyocyte isolation and culture as an illustration of the potential impact of water contamination on tissue culture experiments.
Millipore Corporation
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Cell Culture Workflow Solutions
26 Oct 2007: In this background information note, learn how the new Thermo Scientific cell culture workflow has been developed to cover 5 major areas of specific application-driven processes used in every cell culture laboratory. Also discover the comprehensive range of instrumentation available for each of these areas.
Thermo Fisher Scientific
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MISP – Metrohm Inline Ultrafiltration – Cross Contamination < 0.1%
12 Jun 2007: In this application note, the cross contamination of 100 mg/L of fluoride, chloride, nitrite, bromide, nitrate, phosphate and sulfate to ultrapure water are determined using anion chromatography with conductivity detection.
Metrohm AG
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Filtration of Dilute Protein and DNA Solutions
05 Feb 2007: While heat sterilization is used for many applications, heat may destroy critical cell culture media components and biomolecules needed for normal cell growth. Consequently, filtration is the method of choice for the sterilization of cell culture media. This application note tests the filtration of extremely dilute protein or DNA solutions under dynamic filtration conditions.
Pall Life Sciences Products - Biopharmaceutical Division
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AcroPrep™ 96 Filter Plates Reduce the Presence of False Positive Results in Serological Based Immunoassays with Luminex® xMAP® Technology
 30 Jan 2007: This poster demonstrates that the Pall Life Sciences AcroPrep 96 filter plates with 1.2 µm Supor® membrane show a significant reduction in the presence of false positives compared to competitive filter plates when used with the Luminex xMAP technoloy for serological immunoassays.
Pall Life Sciences Products - Biopharmaceutical Division
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Diafiltration: A Fast, Efficient Method for Desalting, or Buffer Exchange of Biological Samples
28 Dec 2006: Diafiltration is a technique that uses ultrafiltration membranes to completely remove, replace, or lower the concentration of salts or solvents from solutions containing proteins, peptides, nucleic acids, and other biomolecules. This application note discusses the benefits and various uses of diafiltration.
Pall Life Sciences Products - Biopharmaceutical Division
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Testing ZORBAX GF-250 Performance Using Gel-Filtration Test Mixtures
06 Oct 2006: Gel-Filtration standards are used to define the performance and linear separation range of gel-filtration columns. This application note demonstrates the use of a different set of compounds to characterise the ZORBAX GF-250 column.
Agilent Technologies
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